Attempts to increase support by busing in supporters also appeared to fail to muster the desired response. Research by GB News showed most buses could still be booked just hours before the march officially began.
The protesters walked to Parliament Square in Westminster
Notable figures including 'Stop Brexit Man' Steve Bray were in attendance
"I want freedom for my future, and future generations as well."
And an EU national in attendance who gave their name only as Eugene added: "I moved here in 2014 and I remember that this country was much better organised and things were going much smoother when we were int he EU and i don't see that happening after Brexit to be honest.
"I know you will say that I am biased, coming from an EU country, but I've lived here for more than nine years so I can tell the difference."
Eugene said that he believed the cost of living crisis currently felt by Britons was in "a great part of it due to the referendum".
The failure of the pro-EU march to attract anywhere near as many attendees as in the past comes as polling shows there is little appetite for a new Brexit referendum in the immediate future.
A YouGov poll of 2,101 Britons conducted between August 8 and 9 found that just 26 per cent of people would want a referendum this year.
Beth Mann, a political researcher at YouGov, said at the time: "Despite high levels of Bregret among the British public, this new polling shows that there is not necessarily a widespread appetite for another referendum any time soon.
"However, there are strong party divisions that could swing campaigns as we head into the next election and beyond, with a significant majority of those who currently intend to vote Labour backing a referendum on rejoining the EU within the next five or 10 years."