This time around, Cooper and a friend who also owns dairy cows underwent the testing together.
Cooper and his pal's 240 cows were tested on Thursday afternoon but just when he thought he'd gotten through the testing unscathed, the Clarkson's Farm star was informed of a reactor.
Jeremy Clarkson's righthand man explained all as he spoke directly to the camera in a new video in which he said: "So TB testing today where I keep my dairy cows as well as a friend of mine.
"(I'm) helping them to run 240 cows through the crush," he continued. "On the last four, one had a reactor - one out of 240."
Kaleb Cooper has become a household name for starring in Clarkson's Farm
Cooper struggled to keep his composure as he went on: "I mean, the feeling... I can't really describe the feeling when the vet says, 'Kaleb your reactor...'
"To anyone out there - sorry I can't even speak now," Cooper said as he became choked up. "But to anyone out there who's got TB problems, I feel you.
"I feel you and I'm with you," he reiterated before the clip drew to a close.
While it was only one reactor which will have to be destroyed, the impact of one amongst even as many as 240 cows could be catastrophic.
Cooper's herd will now be subject to strict movement restrictions after one reactor has been detected.
According to the government's Animal and Plant Health Agency (APHA): "Your herd will be put under movement restrictions and its OTF (Officially TB Free) status will be suspended making it OTFS.
"If your herd status is suspended because of suspicion of TB in an animal at a slaughterhouse inspection, you’ll need to get a negative culture result and clear check test before your herd can regain its OTF status.
"If the suspected animal has a positive culture result, or any TB skin test reactor is found, your herd will become a breakdown herd."