Iranian footballers and team officials held photographs of children they claim were killed by American and Israeli airstrikes ahead of their World Cup preparation fixture against Costa Rica on Tuesday.
The demonstration took place in Antalya, southern Turkey, with players standing alongside head coach Amir Ghalenoei and Iran Football Federation vice president Mehdi Mohammad Nabi during the national anthem.
Staff members also participated in the display, clutching the images as the anthem played out in the empty stadium.
Fifa President Gianni Infantino attended the match despite no spectators being permitted entry to the venue.
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This gesture followed a similar tribute on Friday, when the squad held small backpacks before their warm-up fixture against Nigeria to commemorate those who perished in a devastating missile strike on a primary school in southern Iran.
The attack on February 28 claimed more than 165 lives, with the majority of victims being children.
Evidence suggests the strike was most likely carried out by the United States.
The incident has drawn widespread condemnation from the United Nations and various human rights organisations.
Iranian footballers and team officials held photographs of children they claim were killed by American and Israeli airstrikes
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Washington has denied responsibility and maintains its forces would never deliberately target civilian populations, with the US military currently conducting an investigation into the attack.
Iran claimed a comprehensive 5-0 victory in the fixture, with the Fifa chief watching on from the stands.
The stadium remained closed to supporters throughout the encounter.
Israel has similarly refused to accept blame for the elementary school attack that prompted the players' memorial gestures.
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The protest took place ahead of their World Cup preparation fixture against Costa Rica on Tuesday
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Both nations have faced significant international criticism over the incident, though neither government has acknowledged involvement in the strike that devastated the educational facility.
The US military has stated it is examining the circumstances surrounding the attack, reiterating that American forces do not intentionally strike civilian targets.
Iranian officials have expressed their intention to participate in the World Cup, which will be jointly hosted by the United States, Canada and Mexico this June, despite the ongoing conflict.
However, they have stated that travelling to America is not feasible given the war initiated by the US and Israel.
Infantino has resisted efforts to relocate World Cup fixtures, stating that Fifa wishes the tournament to proceed according to the original schedule
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The Iranian national team is scheduled to contest three group-stage matches on American soil.
Iran's ambassador in Mexico City has revealed that the country has requested Fifa relocate these fixtures to Mexico.
It follows comments from President Donald Trump who discouraged the team from attending and raised safety concerns.
Infantino has resisted these efforts, stating that Fifa wishes the tournament to proceed according to the original schedule.
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