Lady Louise was not present alongside her parents, the Duke and Duchess of Edinburgh, as the Royal Family gathered for Trooping the Colour on Saturday.
The 21-year-old royal was notably absent from the proceedings to mark King Charles’s official birthday despite attending last year.
Also missing from the proceedings was Louise’s younger brother James, Earl of Wessex.
The siblings have missed a number of recent royal engagements due to the demands of their academic commitments.
Lady Louise was absent from Saturday's Trooping the Colour celebrations.
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Louise is currently in her third year of an English degree at St Andrews University in Scotland while her brother is working hard on his A-levels.
Her absence at Saturday’s Trooping the Colour marks the third time Louise was forced to miss a recent event.
The 21-year-old was forced to drop out of May's VE Day engagements and the Royal Family's annual Easter celebrations due to her university career.
She is fully dedicated to her coursework as exams at the prestigious university began on May 10.
Louise had missed several recent royal engagements due to her academic commitments.
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St Andrews holds royal significance as the institution where the Prince and Princess of Wales met in 2001.
Lady Louise has embraced a quiet university life, deliberately maintaining a low profile and avoiding unwanted attention.
Beyond her academic studies, Lady Louise has immersed herself in university extracurricular activities.
She joined the St Andrews University Officers' Training Corps, where she has received basic weapons training and leadership lessons.
Sophie rode in a carriage alongside Vice Admiral Sir Timothy Laurence on Saturday.
PAThe royal has also participated in several student productions during her time at university.
Lady Louise is widely known to be considering a career in either the military or diplomacy after graduation.
Louise’s mother and father, Sophie, Duchess of Edinburgh and Prince Edward, King Charles’s younger brother were present for Trooping the Colour.
Sophie rode alongside the husband of Princess Anne, Vice Admiral Sir Timothy Laurence, in a carriage during the procession from Buckingham Palace.
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The Duke of Edinburgh rode alongside Princess Anne in the procession.
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The Duke of Edinburgh rode alongside Princess Anne in the procession.
He was sporting the green sash of the Scots Guards and a bearskin hat along with his red military uniform.
Prince Edward also wore a black armband, a late addition to uniformed royals to commemorate Thursday's Air Indian disaster.
A minutes silence was also observed during the event.
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