Overnight workers could pay two Ulez fines on the same shift, industry chiefs have warned Sadiq Khan.
The London Mayor expanded his controversial carbon-curbing policy to the capital’s outer boroughs.
The decision will slap the £12.50 levy on millions more motorists, including overnight workers.
The Night Time Industries Association ramped up pressure on Khan to rectify “this assault on night-time workers”.
Sadiq Khan has been slammed for putting an Ultra Low Emissions Zone (Ulez) camera next to a cemetaryGB News
NTIA represents businesses that operate between 6pm and 6am.
It estimates nearly 40,000 workers who earn their bread after midnight will need to pay the charge twice during their commute.
Overnight workers, who are often more reliant on cars for travel due to reduced public transport services, could cough up a total of £25.
Michael Kill, the chief executive of NTIA, told The Telegraph: “The midnight threshold unfairly condemns countless night workers who are unable to afford compliant vehicles to a double whammy of charges as they diligently fulfil their duties during unconventional hours.
The Ulez is expanding on August 29 PA
“It also brings into question how many night workers are paying double when public transport is limited due to industrial action.”
A spokesperson for the NTIA added: “As a relentless advocate for the interests of businesses and communities, the NTIA demands an immediate and thorough reevaluation of these draconian measures.
“Collaboration with stakeholders across sectors is imperative to rectify this assault on workers, residents, and visitors alike.”
A leading trade union official raised concerns about the impact of Ulez on overnight workers earlier this year.
An image of Sadiq KhanPA
Unite regional officer Joe McGowan said: “Aviation workers operate 24/7, 365 days a year.
"Shifts begin and end at all hours of the night and it is often not possible to get to work without a car.
“If Ulez is not delayed and then introduced without strong mitigation for workers at Heathrow, then this will be disproportionately unfair for aviation workers and will be a strong disincentive for people seeking work at Heathrow.”
A TfL spokesperson also said: “An integrated Impact Assessment published by Jacobs looked at the impact of the expanded zone on those working at night and the report formed part of the Mayor’s decision when considering expanding the zone.
“As things stand, with the night tube and night buses available and nine out of ten cars seen in the extended zone already compliant, only a very small number of night workers who drive are likely to be affected.”