Harry Maguire has been given an additional one-match suspension and a financial penalty by the FA for directing abusive language at fourth official Matt Donohue following his dismissal during United's encounter with Bournemouth last month.
The ban rules the Manchester United defender out of the anticipated trip to Stamford Bridge this weekend.
The FA charged the 33-year-old England international on April 1, alleging "the defender acted in an improper manner and/or abusive and/or insulting words and/or behaviour towards the fourth official following his dismissal."
Referee Stuart Attwell had shown Maguire a straight red card for denying Evanilson a clear goalscoring opportunity, a suspension he served during Monday's 2-1 home defeat to Leeds United.
TRENDING
Stories
Videos
Your Say
United's defensive woes are compounded by Lisandro Martinez's expected absence after the Argentine was dismissed during the Leeds match.
The 28-year-old received a straight red card when VAR official John Brooks instructed referee Paul Tierney to review footage of Martinez pulling Dominic Calvert-Lewin's hair.
United have lodged an appeal, arguing their defender did not pull with force and was merely competing for the ball.
However, the PGMO remains confident the decision was correct.
Harry Maguire has been given an additional one-match suspension and a financial penalty by the FA
|PA
Howard Webb, the PGMO chief, has previously said on the organisation's Mic'd Up programme that hair pulling constitutes violent conduct under guidance issued to clubs before the season commenced.
Webb explained: "Grabbing someone's hair with force is deemed as violent conduct and a player will be sent off."
Michael Carrick now faces a significant selection headache at Chelsea, with both senior centre-backs unavailable and Matthijs de Ligt still recovering from a back injury that has kept him out since November's defeat at Crystal Palace.
The interim manager will likely be forced to deploy a youthful defensive partnership of Leny Yoro, 20, and 19-year-old Ayden Heaven at Stamford Bridge.
LATEST SPORT NEWS
Harry Maguire has been punished for directing abusive language at fourth official Matt Donohue
|GETTY
Maguire's absence represents a particular blow for Carrick, who has relied heavily on the experienced defender since taking charge in January.
The 33-year-old has started 10 of his 14 Premier League appearances this season under the former United midfielder's stewardship.
Saturday's fixture carries significant implications for United's European ambitions.
Carrick's side currently occupy sixth position in the Premier League, with Chelsea sitting third.
The ban rules the Manchester United defender out of the anticipated trip to Stamford Bridge this weekend
|GETTY
A victory at Stamford Bridge would boost United's Champions League qualification hopes considerably.
Conversely, defeat would see the gap between the two clubs shrink to just four points.
The timing of the defensive crisis could hardly be worse for United, who must now rely on their inexperienced centre-back pairing against one of the division's strongest sides.
Earlier this season, Everton's Michael Keane was also penalised for pulling the braided hair of Wolverhampton Wanderers forward Tolu Arokodare during a 1-1 draw at the Hill Dickinson Stadium.
Our Standards: The GB News Editorial Charter