The Scottish Parliament will hold a no confidence vote on the Scottish Government on Wednesday.
Scottish Labour tabled the motion which, if passed, would mean all Scottish ministers are required to stand down.
The Scottish Tories and Lib Dems have backed the motion but without support from the Greens it would be defeated.
It comes as Scottish Conservatives are withdrawing their motion of no confidence in Humza Yousaf, declaring “job done” after the First Minister announced his intention to resign.
Humza Yousaf has announced he intends to resign
PAThe party has said Holyrood should instead hear from the Lord Advocate on the Post Office Horizon scandal on the time provisionally set aside for their motion on Wednesday.
Scottish Conservative leader Douglas Ross said: "I’m delighted that the Scottish Conservative motion of no confidence in Humza Yousaf achieved its purpose by forcing him to resign. While, on a personal level, I wish him well for the future, he was a disaster as First Minister and it’s in Scotland’s interests that he goes.
"The next goal for my party is to see off this feuding, failing SNP government and switch the focus away from their independence obsession and on to the public’s real priorities – such as growing the economy and improving Scotland’s ailing public services.
"As it’s job done in terms of Humza Yousaf, there’s no longer any need for us to press ahead with a debate on our no-confidence motion."
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Scottish Conservative leader Douglas RossPAScottish Labour leader Anas Sarwar said he wanted to use the motion to highlight the need for a Holyrood election following the turmoil in the SNP. He said the “genie was out of the bottle” for the SNP, adding: “I think this is a dysfunctional, chaotic, divided political party.”
In a meeting of Holyrood’s parliamentary bureau on Tuesday, the Labour motion against the Government was confirmed for Wednesday afternoon.
Arguing the Government was "incompetent" and could not be saved by a new leader, Mr Sarwar said: "We will not be withdrawing the motion.
"I think the Greens and the SNP obviously have already made it clear they would not support such a motion but the principle of that motion still stands. I have no confidence in this SNP Government."
Scottish Labour leader Anas Sarwar
PAGreens MSP Gillian Mackay has said the confidence vote was a tool to "embarrass" Yousaf further, and accused Labour of "parliamentary game-playing."
She said: "Like the withdrawn Tory motion, the Labour one has clearly been overtaken by events. Pursuing it would achieve nothing, and would simply mean more parliamentary game-playing.
"Labour MSPs have spent the last few days saying the government needs to get back to running the country, so why do they want to waste the valuable time of the Scottish Parliament, staff and MSPs by carrying on with this charade when it has no chance of passing?"