Parents at a Church of England primary school claim a trans supply teacher told students as young as seven: "I'm so gorgeous because I've put makeup on and had a shave."
Students were reportedly left "confused" when their teacher described their gender identity at the beginning of the class.
Bitterne Church of England Primary School in Southampton said they were not told the topic would be discussed in class.
A number of parents have complained to the school following the controversial conversation.
Parents at a Church of England primary school claim a trans supply teacher told students as young as seven: 'I'm so gorgeous because I've put makeup on and had a shave'
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"I think it's a bit much for Year 2 children and can be very confusing for them," Rachel Bennet, whose daughter was in the class told the Daily Mail.
"It seems to have caught everyone off guard, particularly when my daughter was in an English lesson and the teacher is talking about their gender and wearing women's clothing.
"We have strong religious values, and you have to question whether it's something the children need to be exposed to at this age."
Headteacher Jen Johnson responded to the complaints from parents in a letter.
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It said: "I want to make it very clear that whilst we were not aware that the supply teacher would be sharing this information related to their gender identity ahead of teaching the lessons, we are a tolerant and respectful school.
"Moving forward, and having spoken to the supply agency, we have reaffirmed the school's expectations for what we expect supply teachers to deliver."
A spokesperson for Southampton City Council said: "Our schools, including Bitterne C of E Primary School, use supply teacher agencies who apply rigorous checks to deliver high quality teaching staff and safeguard students.
Students were reportedly left "confused" when their teacher described their gender identity at the beginning of the class
GETTY"The supply teacher in question is well regarded and the quality of the teaching was of a high standard.
"In classroom, the supply teacher introduced themselves and then responded to a small number of questions about their gender quickly and without detail, before moving on swiftly to deliver the lesson in a professional manner.
"Bitterne C of E Primary School, like many schools in Southampton, has a policy of inclusion and the school has written to parents and the letter has been positively received, with many parents offering supportive feedback."