The closures will take place in early 2024 - in February and March.
Barclays said the number of customers visiting branches has reduced, with "many" customers choosing to bank via the app, Online, or Telephone Banking.
A Barclays spokesperson said: “As visits to branches continue to fall, we need to adapt to provide the best service for all our customers.
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Barclays said the number of customers visiting branches has reduced
"Where there is no longer enough demand to support a branch, we maintain an in-person presence though our Barclays Local network, live in over 300 locations, based in libraries, town halls, mobile vans and our banking pods.
"We also support access to cash with our cashback without purchase service, 24-hour deposit-taking ATMs and by working alongside the Post Office and Cash Access UK.”
Barclays has launched a network of alternative branch formats, known as Barclays Local, present in more than 300 locations.
Barclays Local sites have popped up in places such as town halls and libraries, mobile vans and banking pods.
Full list of 16 Barclays bank branches closing in early 2024: